RNA Polymerases
E. coli Poly(A) Polymerase, 500 U, G-Biosciences
Enzyme that catalyzes the addition of adenosine monophosphate (AMP) residues from adenosine triphosphate (ATP) to the 3' end of RNA molecules, creating a polyadenylated tail.
Cid1 Poly (U) Polymerase, 60 U, G-Biosciences
Cid1 Poly (U) Polymerase is a template-independent RNA polymerase that catalyzes the addition of uridine monophosphates (UMP) to the 3′ end of RNA using UTP as a substrate.
New England Biolabs, Inc. Hi-T7™ RNA Polymerase (High Concentration) – 50000 units
Hi-T7 RNA Polymerase (High Concentration) is offered at a 20-fold higher concentration than our standard Hi-T7 RNA Polymerase and is ideal for experienced users interested in building and optimizing their own in vitro transcription reactions. The enzyme is accompanied by Hi-T7 RNA Polymerase Reaction Buffer and Magnesium Chloride Solution to further enable testing of different conditions such as nucleotide concentrations. In vitro transcription involves multiple components in addition to the RNA Polymerase including ribonucleotides, inorganic pyrophosphatase and RNase Inhibitor, which can be purchased separately. Please refer to the Related Products section for details. Hi-T7 RNA Polymerase is an engineered DNA-dependent RNA polymerase that is highly specific for T7 phage promoters and is designed to function at higher temperatures than the wild-type bacteriophage T7 RNA Polymerase. Hi-T7 RNA Polymerase functions at an optimal temperature of 50-52°C.
Aldevron T7 RNA Polymerase, 2000ug
2000ug dispense of wild type T7 RNA Polymerase. T7 RNA Polymerase catalyzes synthesis of RNA in the presence of a DNA template containing the T7 phage promoter.
ABclonal Technology Phospho-POLR2A CTD-T4 Rabbit pAb
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
SAPPHIRE NORTH AMERICA T7 3M RNA POLYMERASE HC 50UL
T7 3M RNA Polymerase HC Highly concentrated triple mutant T7 RNA Polymerase for set-up of high yield in vitro transcription reactions 50UL
New England Biolabs, Inc. T7 RNA Polymerase (High Concentration) – 50000 units
T7 RNA Polymerase (High Concentration) is offered at a 20-fold higher concentration than our standard T7 RNA Polymerase and is ideal for experienced users interested in building and optimizing their own in vitro transcription reactions. The enzyme is accompanied by RNA Polymerase Reaction Buffer and magnesium chloride solution to further enable testing of different conditions such as nucleotide concentrations. For standard RNA synthesis and high yield reactions, we recommend our T7 RNA Polymerase and HiScribe kits. The HiScribe kits are specifically designed to produce high quality, high yield RNA in a short time with minimal optimization. In vitro transcription involves multiple components in addition to T7 RNA Polymerase including ribonucleotides, inorganic pyrophosphatase and RNase Inhibitor, which can be purchased separately. Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters.
Aldevron Codex HiCapRNA Polymerase, 25uL
25uL dispense of HiCap T7 RNA Polymerase. Codex HiCap RNA Polymerase is a co-transcriptional capping RNA polymerase engineered to produce synthetic RNA to meet increasing demands for safety and cost-effectiveness. The HiCap enzyme generates fewer undesirable double-stranded RNA (dsRNA) byproducts than wild-type T7 (WT T7) RNA polymerase, reducing unwanted immunogenicity, while efficiently incorporating commercially available cap analogs.
New England Biolabs, Inc. E.coli Poly (A) Polymerase – 100 units
Poly(A) Polymerase catalyzes the template independent addition of AMP from ATP to the 3 end of RNA.
INTACT GENOMICS INC ig-Fusion™ Cloning Enzyme Premix 10 Reactions
Intact Genomics propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase or other high fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step. Cloning takes usually 10-30 min. with more than 95% cloning efficiency.Applications Clone any insert at any site within any vector Restriction enzyme and phosphatase free system Joining multiple large fragments at once Precise insertion at a desired orientation Rapid and high efficiency with > 95% positive clones
AURORA BIOLABS LLC T7 RNA High Yield Synthesis Assay, 100 rxn
Bacteriophage T7 RNA Polymerase is a 99 kDa protein that recognizes T7 phage promoters with high specificity and subsequently initiates transcription. T7 RNA polymerase is a single subunit, highly processive and stable enzyme, characteristics that make it suitable for a broad range of biochemistry and molecular biology applications. Bacteriophage T7 RNA Polymerase is a 99 kDa protein that recognizes T7 phage promoters with high specificity and subsequently initiates transcription. T7 RNA polymerase is a single subunit, highly processive and stable enzyme, characteristics that make it suitable for a broad range of biochemistry and molecular biology applications. The assay is fast and convenient, and requires the T7 RNA polymerase, NTP mix (UTP, ATP, CTP, and GTP), reaction buffer, and a suitable DNA template. The modified nucleotide N1- Methyl-Pseudo UTP is incorporated in our T7 RNA Polymerase In Vitro Transcription Kit-II.